Rheumatoid Arthritis (RA) is a chronic, progressive systemic autoimmune disease characterised by persistent joint inflammation. If left untreated, the disease leads to systemic complications, irreversible joint damage and invalidity. Serological markers can detect early stage RA to lead to improved outcome of RA therapy and reduction of severity of joint damage.
AUTOZYME® RF is for the quantitative detection of IgA, IgM or IgG Rhuematoid Factors in human sera or plasma to aid the diagnosis of rheumatoid arthritis in conjunction with other laboratory tests and clinical findings.
The AUTOZYME™ method employs antigen-coated microwell technology, which is ideal for batch screening of large and small numbers of samples for rheumatoid factor IgA, IgM and IgG. The method employs a sandwich enzyme immunoassay (EIA) principle.
- Antigen: horse IgG
- Calibration: 5 calibrators (quantitative), related to WHO W1066 for Rheumatoid Factor which replaced the 1st British Standard 64/2.
- Serum samples, Dilution: 1/100 (10μl sample+ 990μl diluent)
- No interference from Bilirubin, Haemoglobin, Lipids, Ascorbic Acid
- Assay Time: 30’, 30’ 30’
Serolgical markers include Rheumatoid Factor (RF) and antibodies against cyclic citrullinated peptides (Anti-CCP).
AUTOZYMETM RF IgA, IgG and IgM Elisa assays provide excellent quantification, ideal for routine use on Elisa processors such as the Dynex DS2.
Z9196: AUTOZYME® RF IgA
Z9296: AUTOZYME® RF IgG
Z9396: AUTOZYME® RF IgM
Download the AUTOZYME® RF datasheet.
CLS also can provide RF and anti-CCP elisa assays and assays that are suitable for clinical chemistry analysers.