Gastrointestinal Autoimmune Disease

Immunoassays for the detection of autoantibodies in patients with autoimmune gastric diseases, particularly celiac disease (CD) and inflammatory bowel disease (IBD).  Serology studies are an important adjunct in diagnosis of IBD.  The combination of ANCA and ASCA provides good discrimination between the two types of IBD, Crohn’s disease and ulcerative colitis. 

Coeliac Disease

The transglutaminase enzyme (tTG) is the target antigen of autoantibodies found in the serum of patients suffering from coeliac disease (CD). Anti-tTG IgA are a very sensitive and specific marker for CD, while anti-tTG IgG can be used to diagnose CD in patients with IgA deficiency. Anti-tTG recognise the same antigen as anti-endomysial antibodies (EMA) which are tested by immunofluorescence on monkey oesophagus slides.  EMA IgA is highly specific but less sensitive than anti-tTG IgA. Anti-gliadin antibodies are also useful to identify patients with CD.

These antibodies are detected by immunofluorescence on mouse or rat stomach tissue or ELISA or BlueDiver Dot

Inflammatory Bowel Diseases – Crohn’s + Ulcerative Colitis

Crohn's Disease is one of the two major Inflammatory Bowel Diseases (IBD). IBD is an umbrella term covering both primary chronic disorders that cause inflammation or ulceration in the small and large intestine, Crohn's Disease and Colitis Ulcerosa.  Anti-Sacchromyces Cerevisiae Antibodies (ASCA) are reported in patients with Crohn's Disease and can be detected using BlueDiver Dot assays.

Autoimmune Gastritis

Autoimmune gastritis is restricted to the parietal cell containing corpus of the stomach. Circulating autoantibodies to gastric parietal cell H+/K+ ATPase is a diagnostic marker for autoimmune gastritis. These antibodies are detected by immunofluorescence on mouse or rat stomach tissue or ELISA or BlueDiver Dot.

Pernicious Anaemia

Pernicious anaemia is accompanied by the development of autoantibody to intrinsic factor, a secretory product of gastric parietal cells. Intrinsic factor autoantibodies, present in serum and gastric juice, can block intrinsic factor from binding vitamin B12 and hinder the absorption by receptors in the ileum.

The AUTOZYME™ IFAB Anti-Intrinsic Factor Antibodies Assay is an easy, rapid and sensitive method allowing the detection of total anti-intrinsic factor antibodies. The use of recombinant intrinsic factor as antigen ensures the specificity of the method.

Product Codes:

Z4396: AUTOZYME™ IFAB - download the datasheet

Autoimmune Thyroiditis

This is a self-destructive process in which the body’s immune system attacks the thyroid cells as though they were foreign cells. Mild thyroiditis may occur in up to 20% of otherwise healthy women (men have a four- to ten-fold lower frequency of thyroiditis). The autoimmune process progresses to cause ill-health in only a small proportion of these people (about 10% of those with thyroiditis). The most common form of autoimmune thyroiditis is known as ‘Hashimoto’s thyroiditis’.

Tests detect the presence and measure the quantity of specific thyroid autoantibodies in the blood. These autoantibodies develop when a person’s immune system mistakenly recognises components of the thyroid gland or thyroid proteins as foreign (non-self) and can lead to chronic inflammation of the thyroid (thyroiditis), tissue damage, and disruption of thyroid function. The main tests are for Thyroid peroxidase antibody (TPOAb); Thyroglobulin antibody (TgAb); Thyroid stimulating hormone receptor antibody (TRAb). Thyroid antibodies can also be found in other autoimmune disorders, such Type 1 diabetespernicious anaemiarheumatoid arthritis, and SLE.

Thyroid peroxidase antibody (TPOAb)

Present in Autoimmune thyroid disease: Hashimoto’s thyroiditis (95%); primary myxoedema (90%); Graves’ disease (18%) and is used when patient has symptoms or test results suggesting hypothyroidism; when a doctor is considering starting a patient on a drug therapy, such as lithium, amiodarone, interferon alpha, or interleukin-2, that has associated risks of developing hypothyroidism when TPOAb are present.

The AUTOZYME™ TAB Anti-Thyroid Peroxidase Antibodies Assay is an easy, rapid and sensitive ELISA assay allowing the detection of anti-thyroid peroxidase (TPO) antibodies. The use of recombinant thyroid peroxidase as antigen ensures the specificity of the method.

Product Codes:

Z2396: AUTOZYME™ TAB Anti-Thyroid Peroxidase Antibodies Assay - download the datasheet.

Thyroglobulin antibody (TgAb)

Tested as part of monitoring of treatment for thyroid cancer.  Also present in autoimmune thyroid disease. Thyroglobulin antibody together with thyroglobulin levels are used at regular intervals after thyroid cancer treatment.

The AUTOZYME™ TAB Anti-Thyroglobulin Antibodies Assay is an easy, rapid and sensitive ELISA assay allowing the detection of anti-thyroglobulin (Tg) antibodies. The use of high purity human thyroglobulin as antigen ensures the specificity of the method.

Product Codes:

Z2196: AUTOZYME™ TAB Anti-Thyroglobulin Antibodies Assay - download the datasheet.

Thyroid stimulating hormone receptor antibody (TRAb)

Is present in Graves’ disease and is used when patient has symptoms of  hyperthyroidism; to monitor effectiveness of anti-thyroid therapy.

The ElisaRSR™ TRAb Fast™ TSH Receptor (TSHR) Autoantibody Elisa Kit is an easy, rapid and sensitive ELISA assay for the determination of autoantibodies to TSH receptor in serum. The total assay time is fast, approx. 2 hours.

Product Codes:

FTE/96: ElisaRSR™ TRAb Fast™ - download the datasheet.

 

Resources

CodeDescriptionMSDSInstructions for use
AD-EMO96Anti-Endomysial IgA (EMA) Test System
FA3403AAS Mouse Kidney/Stomach/Liver Tissue Test System
Z4396AUTOZYME™ IFAB
Z2196AUTOZYME™ TAB Anti Tg
Z2396AUTOZYME™ TAB Anti TPO
5144GImmuLisa™ Celiac (tTG) IgG Antibody Enhanced ELISA
5144AImmuLisa™ Celiac (tTG) IgA Antibody Enhanced ELISA
5159AImmuLisa™ Celiac G+ (Deamidated Gliadin) IgA Antibody Enhanced ELISA
5159GImmuLisa™ Celiac G+ (Deamidated Gliadin) IgG Antibody Enhanced ELISA
FTE/96ElisaRSR™ TRAb Fast™ TSH Receptor Autoantibody ELISA Kit
ENDADIV-24BlueDiver Dot Celiac IgA
ENDGDIV-24BlueDiver Dot Celiac IgG
ASCCDIV-24BlueDiver Dot ASCA IgG +IgA
IFPCADIV-24BlueDiver Dot Gastritis IgG
Top